In the conduct of live-cell, cell transfer or cell traffic, the first essential consideration are the techniques and the experimental design that will be utilized in the study because this will be very important in ensuring that you will have a valid conclusion. The interpretation of studies that is using gynecology microscopes, particularly the inverted tissue culture microscopes and the embryo-transplant microscopes, for the identification of a microchimeric DNA, needs adequate knowledge of the specificity and the sensitivity of the certain tests and assays that will be used and the quantity of the DNA aliquots that already tested and of how many times that particular test was replicated. The use of gynecology microscopes particularly that of inverted tissue culture microscopes and embryo-transplant microscopes could be jeopardized by the cross-reactivity of several Y-chromosome sequences with the corresponding autosomal sequences producing false-positive results in the conduct of live-cell observation and microscopy examination. Nested gynecology microscopy techniques are nonquantitative and it has higher susceptibility to contamination as compared to closed gynecology microscope systems. In spite of very high sensitivity of tests and certain assays that is focusing on Y-chromosome multi-copy sequences, several Y-sequences vary in the quantity of copy number between individual men so that only the multi-copy Y-chromosome sequences that have a secure copy number between men must be utilized for studies that requires quantitative purposes.

Other causes of deviation that will occur relies both on the specific technique that will be utilized on the processing of blood and also on the blood compartment which will be the subject of investigation. Like for in instance, in the occurrence of pregnancy, the DNA of the fetus is much greater in the maternal plasma than the PBMC. On the opposite, a long standing persistence of any foreign cell colonies that is present in an individual’s body as identified under a gynecology microscope can only be found within the cellular component and not in the plasma, based on a study that was conducted employing both women with SSc and those that are healthy individuals. The test for Microchimerism can be affected by the speed of the centrifugation and the utilization of a filtration step which in turn can alter the total DNA that can be collected for the identification of the said condition. Moreover, the extraction of DNA through paraffin-embedded tissues is susceptible to contamination due to the fact that paraffin baths are not changed in between the collection of samples. In addition to that, other technological and technical issues that may arise in the conduct of this particular study if the occurrence of overlapping cells which can produce artifacts, in which the cells that produces two distinct signals and have a well-defined nucleus should be counted.

The most favored design that is used for investigation or study of foreign colonies that is present in a certain individual that can be detected under a gynecology microscope is the potential inclusion of the history of pregnancy and other possible sources of Microchimerism. Some of the investigations that assayed male DNA that is present in a female host have also reported outcomes as fetal foreign cell colonies residing in a certain individual that was identified under a gynecology microscope, but lacked the needed history of pregnancy. The possible sources of male DNA that can be found in women can be attributed to several factors such as prior pregnancy, blood transfusion, twin, sometimes through sexual intercourse, or maybe from a male sibling that has been passed through the maternal circulation. The utilization of assays as tests in the detection of unique genetic polymorphisms would be particularly useful; in conducting studies in the future, such as an investigation that is centering on the proof of the fetal origin of the male DNA. Furthermore, in the stage of disease and the administration of medications, particularly the immunological-active medications at the time of the collection of the blood sample must be analyzed thoroughly for they could confuse the possible linked between the foreign cell colonies that is dwelling in an individual and a certain disease which can be detected under a gynecology microscope.

In the event of pregnancy, there occurs a bi-directional cell traffic between the mother and the child, wherein the foreign cell colonies that is present in an individual continues to live in their respective hosts even decades later, as shown under a gynecology microscopes. Latest studies that are conducting observations on fetal and maternal foreign cell colonies that could be found inside an individual could be identified using a gynecology microscope which further gives evidence for the probability that foreign cell colonies that is dwelling within an individual could add up to the pathogenesis of certain diseases, particularly, that of SSc, neonatal lupus and a condition known as autoimmune thyroid disease. In addition, those cells found out to be Microchimeric could aid in the repair of tissues, thus benefiting its host. Under a gynecology microscope, it could be possible for the detection of the effects of foreign cells living on the body, whether beneficial, neutral or even adverse effects to the host, which could entirely depend on the factors such as HLA genes and the HLA-relationship among the essential cells in the body. Clarifying the exact mechanism in which the naturally acquired foreign cell colonies that is living inside the body through the help of a gynecology microscope are tolerated to live inside the host without harmful effects to the host, may lead to new ideas and strategies in the prevention or in curing autoimmune diseases. An in depth research and investigation on this area of study may become very beneficial in the advancement of the knowledge and understanding in the field of iatrogenic chimerism which is a very essential factor in the conduct of organ and stem cell transplantation.